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Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.

ABSTRACT: We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ?1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.

SUBMITTER: Wang L

PROVIDER: S-EPMC4039345 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.

Wang Liang L Han Yujing Y Zhou Shuo S Wang Guihua G Guan Xiyun X

ACS applied materials & interfaces 20140514 10

We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ∼1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specif ...[more]

PMID: 24806593

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