Nanopore biosensor for label-free and real-time detection of anthrax lethal factor.

ABSTRACT: We report a label-free real-time nanopore sensing method for the detection of anthrax lethal factor, a component of the anthrax toxin, by using a complementary single-stranded DNA as a molecular probe. The method is rapid and sensitive: sub-nanomolar concentrations of the target anthrax lethal factor DNA could be detected in ?1 min. Further, our method is selective, which can differentiate the target DNA from other single-stranded DNA molecules at the single-base resolution. This sequence-specific detection approach should find useful application in the development of nanopore sensors for the detection of other pathogens.

SUBMITTER: Wang L 

PROVIDER: S-EPMC4039345 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature