Ontology highlight
ABSTRACT:
SUBMITTER: Preeti A
PROVIDER: S-EPMC4039722 | biostudies-literature | 2014 Sep
REPOSITORIES: biostudies-literature
Preeti Arivaradarajan A Hemalatha Devaraj D Rajendhran Jeyaprakash J Mullany Peter P Gunasekaran Paramasamy P
Indian journal of microbiology 20140213 3
The human oral metagenomic DNA cloned into plasmid pUC19 was used to construct a DNA library in Escherichia coli. Functional screening of 40,000 metagenomic clones led to identification of a clone LIP2 that exhibited halo on tributyrin agar plate. Sequence analysis of LIP2 insert DNA revealed a 939 bp ORF (omlip1) which showed homology to lipase 1 of Acinetobacter junii SH205. The omlip1 ORF was cloned and expressed in E. coli BL21 (DE3) using pET expression system. The recombinant enzyme was pu ...[more]