Structural characterization of IgG1 mAb aggregates and particles generated under various stress conditions.

ABSTRACT: IgG1 mAb solutions were prepared with and without sodium chloride and subjected to different environmental stresses. Formation of aggregates and particles of varying size was monitored by a combination of size-exclusion chromatography, Nanoparticle Tracking Analysis, Micro-flow Imaging (MFI), turbidity, and visual assessments. Stirring and heating induced the highest concentration of particles. In general, the presence of NaCl enhanced this effect. The morphology of the particles formed from mAb samples exposed to different stresses was analyzed from transmission electron microscopy and MFI images. Shaking samples without NaCl generated the most fibrillar particles, whereas stirring created largely spherical particles. The composition of the particles was evaluated for covalent cross-linki

SUBMITTER: Telikepalli SN 

PROVIDER: S-EPMC4040128 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature