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Effect of sequence features on assembly of spider silk block copolymers.

ABSTRACT: Bioengineered spider silk block copolymers were studied to understand the effect of protein chain length and sequence chemistry on the formation of secondary structure and materials assembly. Using a combination of in vitro protein design and assembly studies, we demonstrate that silk block copolymers possessing multiple repetitive units self-assemble into lamellar microstructures. Additionally, the study provides insights into the assembly behavior of spider silk block copolymers in concentrated salt solutions.

SUBMITTER: Tokareva OS

PROVIDER: S-EPMC4040161 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Effect of sequence features on assembly of spider silk block copolymers.

Tokareva Olena S OS Lin Shangchao S Jacobsen Matthew M MM Huang Wenwen W Rizzo Daniel D Li David D Li David D Simon Marc M Staii Cristian C Cebe Peggy P Wong Joyce Y JY Buehler Markus J MJ Kaplan David L DL

Journal of structural biology 20140312 3

Bioengineered spider silk block copolymers were studied to understand the effect of protein chain length and sequence chemistry on the formation of secondary structure and materials assembly. Using a combination of in vitro protein design and assembly studies, we demonstrate that silk block copolymers possessing multiple repetitive units self-assemble into lamellar microstructures. Additionally, the study provides insights into the assembly behavior of spider silk block copolymers in concentrate ...[more]

PMID: 24613991

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