Ontology highlight
ABSTRACT:
SUBMITTER: Zhao N
PROVIDER: S-EPMC4041034 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
The international journal of biochemistry & cell biology 20140322
To investigate the potential clinical application of aptamers to prevention of HIV infection, single-stranded DNA (ssDNA) aptamers specific for CD4 were developed using the systematic evolution of ligands by exponential enrichment approach and next generation sequencing. In contrast to RNA-based aptamers, the developed ssDNA aptamers were stable in human serum up to 12h. Cell binding assays revealed that the aptamers specifically targeted CD4-expressing cells with high binding affinity (Kd=1.59n ...[more]