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Identification of boron-deficiency-responsive microRNAs in Citrus sinensis roots by Illumina sequencing.


ABSTRACT: BACKGROUND: Boron (B)-deficiency is a widespread problem in many crops, including Citrus. MicroRNAs (miRNAs) play important roles in nutrient deficiencies. However, little is known on B-deficiency-responsive miRNAs in plants. In this study, we first identified miRNAs and their expression pattern in B-deficient Citrus sinensis roots by Illumina sequencing in order to identify miRNAs that might be involved in the tolerance of plants to B-deficiency. RESULTS: We isolated 52 (40 known and 12 novel) up-regulated and 82 (72 known and 10 novel) down-regulated miRNAs from B-deficient roots, demonstrating remarkable metabolic flexibility of roots, which might contribute to the tolerance of plants to B-deficiency. A model for the possible roles of miRNAs in the tolerance of roots to B-deficiency was proposed. miRNAs might regulate the adaptations of roots to B-deficiency through following several aspects: (a) inactivating reactive oxygen species (ROS) signaling and scavenging through up-regulating miR474 and down-regulating miR782 and miR843; (b) increasing lateral root number by lowering miR5023 expression and maintaining a certain phenotype favorable for B-deficiency-tolerance by increasing miR394 expression; (c) enhancing cell transport by decreasing the transcripts of miR830, miR5266 and miR3465; (d) improving osmoprotection (miR474) and regulating other metabolic reactions (miR5023 and miR821). Other miRNAs such as miR472 and miR2118 in roots increased in response to B-deficiency, thus decreasing the expression of their target genes, which are involved in disease resistance, and hence, the disease resistance of roots. CONCLUSIONS: Our work demonstrates the possible roles of miRNAs and related mechanisms in the response of plant roots to B-deficiency.

SUBMITTER: Lu YB 

PROVIDER: S-EPMC4041134 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Identification of boron-deficiency-responsive microRNAs in Citrus sinensis roots by Illumina sequencing.

Lu Yi-Bin YB   Yang Lin-Tong LT   Qi Yi-Ping YP   Li Yan Y   Li Zhong Z   Chen Yan-Bin YB   Huang Zeng-Rong ZR   Chen Li-Song LS  

BMC plant biology 20140507


<h4>Background</h4>Boron (B)-deficiency is a widespread problem in many crops, including Citrus. MicroRNAs (miRNAs) play important roles in nutrient deficiencies. However, little is known on B-deficiency-responsive miRNAs in plants. In this study, we first identified miRNAs and their expression pattern in B-deficient Citrus sinensis roots by Illumina sequencing in order to identify miRNAs that might be involved in the tolerance of plants to B-deficiency.<h4>Results</h4>We isolated 52 (40 known a  ...[more]

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