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ABSTRACT:
SUBMITTER: Eisenreich W
PROVIDER: S-EPMC404119 | biostudies-literature | 2004 Apr
REPOSITORIES: biostudies-literature
Eisenreich Wolfgang W Ettenhuber Christian C Laupitz Ralf R Theus Cornelia C Bacher Adelbert A
Proceedings of the National Academy of Sciences of the United States of America 20040419 17
Drosophila melanogaster strain Oregon-R(*) was grown on standard medium supplemented with [U-(13)C(6)]glucose. One to two days after hatching, flies were extracted with water. Glucose was isolated chromatographically from the extract and was analyzed by (13)C NMR spectroscopy. All (13)C signals of the isolated glucose were multiplets arising by (13)C(13)C coupling. Based on a comprehensive analysis of the coupling constants and heavy isotope shifts in glucose, the integrals of individual (13)C s ...[more]