Ontology highlight
ABSTRACT:
SUBMITTER: Florian AE
PROVIDER: S-EPMC4043149 | biostudies-literature | 2013 Apr
REPOSITORIES: biostudies-literature
Florian Amy E AE Lepensky Christopher K CK Kwon Ohyun O Haynes Mark K MK Sklar Larry A LA Zweifach Adam A
Journal of biomolecular screening 20121115 4
We developed a homogeneous phenotypic fluorescence end-point assay for cytotoxic T lymphocyte lytic granule exocytosis. This flow cytometric assay measures binding of an antibody to a luminal epitope of a lysosomal membrane protein (LAMP-1) that is exposed by exocytosis to the extracellular solution. Washing to remove unbound antibody is not required. Confirming the assay's ability to detect novel active compounds, we screened at a concentration of 50 µM a synthetic diversity library of 91 compo ...[more]