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Detection of Candida albicans mRNA in archival histopathology samples by reverse transcription-PCR.

ABSTRACT: The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age.

SUBMITTER: Beggs KT

PROVIDER: S-EPMC404655 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature

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Detection of Candida albicans mRNA in archival histopathology samples by reverse transcription-PCR.

Beggs Kyle T KT Holmes Ann R AR Cannon Richard D RD Rich Alison M AM

Journal of clinical microbiology 20040501 5

The feasibility of detecting Candida albicans mRNA in formalin-fixed paraffin-embedded archival human histopathology specimens by reverse transcription-PCR (RT-PCR) was investigated. RT with gene-specific primers was used to detect five single-copy C. albicans gene transcripts, including those of two housekeeping genes, in oral candidiasis samples up to 8 years of age. ...[more]

PMID: 15131211

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