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Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.

ABSTRACT: Many studies have reported the presence of bacterial DNA contamination in commercial Taq DNA polymerase reagents. This is the first report of the presence of phage-like DNA sequences in certain commercial Taq DNA polymerase reagents. Precautions are needed when using amplification reagents with exogenous DNAs.

SUBMITTER: Newsome T

PROVIDER: S-EPMC404674 | biostudies-literature | 2004 May

REPOSITORIES: biostudies-literature

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Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.

Newsome Tamara T Li Bing-Jie BJ Zou Nianxiang N Lo Shyh-Ching SC

Journal of clinical microbiology 20040501 5

Many studies have reported the presence of bacterial DNA contamination in commercial Taq DNA polymerase reagents. This is the first report of the presence of phage-like DNA sequences in certain commercial Taq DNA polymerase reagents. Precautions are needed when using amplification reagents with exogenous DNAs. ...[more]

PMID: 15131208

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