Bovine mammary epithelial cell cultures for the study of mammary gland functions.

ABSTRACT: In the present study, the analysis of epithelial cells derived from various sources was undertaken, beginning from the mammary gland tissue through the primary cultures and their subsequent passages. The objective of the study was the comparative analysis of the stage in which the epithelial cells obtained from individuals in different lactation cycles and disparate phases of cell culture growth are the most suitable for morphological research and analysis of gene expression activity. The cultures of primary bovine mammary epithelial cells and passages were identified morphologically using immunocytochemical methods. After positive identification, real-time PCRs were performed for the analysis of the expression level of casein genes, whey protein genes, and butyrophilin gene. The most stab

SUBMITTER: Jedrzejczak M 

PROVIDER: S-EPMC4047483 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature