Dataset Information

Association between the melanopsin gene polymorphism OPN4*Ile394Thr and sleep/wake timing in Japanese university students.

ABSTRACT: BACKGROUND: In our previous studies, we found that the Ile394Thr SNP in the melanopsin gene (OPN4) was functionally associated with the pupillary light reflex. This indicates the possibility that OPN4*Ile394Thr is associated with other non-image forming responses. The aim of this study was therefore to determine whether OPN4*Ile394Thr is associated with sleep/wake timing. METHODS: A total of 348 healthy Japanese university students participated in this study. Scalp hair was used to genotype the Ile394Thr SNP of OPN4. Sleep habits, including bedtime, wake time and sleep duration, were assessed separately for weekdays and weekends. A total of 328 samples, including 223 samples with TT genotype, 91 with TC genotype and 14 with CC genotype, were used for statistical analysis. No significant difference in age or male/female distribution was found among the three genotype groups. RESULTS: There was no significant difference in circadian preference among the genotype groups. During weekdays, bedtime, wake time and midpoint of sleep for CC subjects were significantly later than those for TT and TC subjects. However, there was no difference between TT and TC subjects in any of their sleep habits. During weekends, bedtime of CC subjects was significantly later than those of TT and TC subjects, and the midpoint of sleep of CC subjects was significantly later than that of TC subjects. CONCLUSIONS: Our findings demonstrated that OPN4*Ile394Thr is associated with sleep/wake timing. We also found that the sleep/wake timing of subjects with the CC genotype was later than that of subjects with the TT or TC genotype.

SUBMITTER: Lee SI

PROVIDER: S-EPMC4048048 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Association between the melanopsin gene polymorphism OPN4*Ile394Thr and sleep/wake timing in Japanese university students.

Lee Sang-Il SI Hida Akiko A Kitamura Shingo S Mishima Kazuo K Higuchi Shigekazu S

Journal of physiological anthropology 20140512

<h4>Background</h4>In our previous studies, we found that the Ile394Thr SNP in the melanopsin gene (OPN4) was functionally associated with the pupillary light reflex. This indicates the possibility that OPN4*Ile394Thr is associated with other non-image forming responses. The aim of this study was therefore to determine whether OPN4*Ile394Thr is associated with sleep/wake timing.<h4>Methods</h4>A total of 348 healthy Japanese university students participated in this study. Scalp hair was used to ...[more]

PMID: 24887407

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Project description:Light influences sleep and alertness either indirectly through a well-characterized circadian pathway or directly through yet poorly understood mechanisms. Melanopsin (Opn4) is a retinal photopigment crucial for conveying nonvisual light information to the brain. Through extensive characterization of sleep and the electrocorticogram (ECoG) in melanopsin-deficient (Opn4(-/-)) mice under various light-dark (LD) schedules, we assessed the role of melanopsin in mediating the effects of light on sleep and ECoG activity. In control mice, a light pulse given during the habitual dark period readily induced sleep, whereas a dark pulse given during the habitual light period induced waking with pronounced theta (7-10 Hz) and gamma (40-70 Hz) activity, the ECoG correlates of alertness. In contrast, light failed to induce sleep in Opn4(-/-) mice, and the dark-pulse-induced increase in theta and gamma activity was delayed. A 24-h recording under a LD 1-hratio1-h schedule revealed that the failure to respond to light in Opn4(-/-) mice was restricted to the subjective dark period. Light induced c-Fos immunoreactivity in the suprachiasmatic nuclei (SCN) and in sleep-active ventrolateral preoptic (VLPO) neurons was importantly reduced in Opn4(-/-) mice, implicating both sleep-regulatory structures in the melanopsin-mediated effects of light. In addition to these acute light effects, Opn4(-/-) mice slept 1 h less during the 12-h light period of a LD 12ratio12 schedule owing to a lengthening of waking bouts. Despite this reduction in sleep time, ECoG delta power, a marker of sleep need, was decreased in Opn4(-/-) mice for most of the (subjective) dark period. Delta power reached after a 6-h sleep deprivation was similarly reduced in Opn4(-/-) mice. In mice, melanopsin's contribution to the direct effects of light on sleep is limited to the dark or active period, suggesting that at this circadian phase, melanopsin compensates for circadian variations in the photo sensitivity of other light-encoding pathways such as rod and cones. Our study, furthermore, demonstrates that lack of melanopsin alters sleep homeostasis. These findings call for a reevaluation of the role of light on mammalian physiology and behavior.

Project description:BackgroundProcrastination is a psychological trait that causes individuals to put off doing things that need to be done. It has recently shown to result in the worsening of symptoms due to delays in seeking medical care. However, it is not clear how perception of dental disease influences dental visits. This study examined the associations of procrastination and personality traits with delayed dental visits for both acute and chronic conditions.MethodsOf 599 university students queried, the data of 549 subjects (mean age 19.7 years) were analyzed. A general procrastination scale (GPS), the Big Five personality traits, and oral hygiene habits were used for analysis. The participants were asked about illness awareness conditions related to dental disease, perception of pain in the oral region due to acute oral symptoms and chronic symptoms. The participants were asked the number of days until they decided that treatment was required. Based on the bimodal shape of the distribution, those who answered at least eight days for acute or chronic conditions were classified as the procrastination (P) group and the others as the non-procrastination (Non-P) group.ResultsSignificant differences in GPS scores were found between the groups for both acute and chronic conditions, with significant differences in the Big Five traits of extraversion, agreeableness, and neuroticism for an acute condition and extraversion, openness, and neuroticism for a chronic condition. There were no significant differences regarding oral hygiene habits between the groups for either condition. Next, using a Bayesian network, the probabilistic causal relations among procrastination, the Big Five traits, and delays in dental visits for both acute and chronic conditions were analyzed. Among the Big Five traits, conscientiousness and neuroticism were directly related to GPS score. Interestingly, agreeableness was directly related to delays in dental visits only for an acute condition and showed a negative effect, while dental student status had a positive effect on delays in dental visits.ConclusionsThe results showed that procrastination and dentistry department are factors that directly influence delays in dental visits, while agreeableness, a Big Five trait, has a negative effect on individuals with an acute condition.

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Dataset Information

Association between the melanopsin gene polymorphism OPN4*Ile394Thr and sleep/wake timing in Japanese university students.

Publications

Association between the melanopsin gene polymorphism OPN4*Ile394Thr and sleep/wake timing in Japanese university students.

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