Molecular and ultrastuctural changes of rat pre-implantation embryos during two-cell developmental arrest.

ABSTRACT:

Background

Rat pre-implantation embryos often suffer 2-cell stage developmental arrest and fail to progress further under in-vitro conditions.

Objective

In order to understand underlying mechanism leading to 2-cell arrest, we investigated the molecular changes, culture conditions and subcellular changes.

Methods

Gene expression in in-vivo developed 2-cell embryos (in-vivo), in- vitro developed 2-cell embryos (in-vitro), and in-vitro 2-cell arrested embryos (arrested) were investigated using microarrays and real-time PCR. Ultra-structural changes were determined using electron microscopy.

Results

Gene expression was similar between in-vivo and in-vitro embryos. Over 2400 genes changed in arrested embryos compared to in-vivo and in-vitro embryos. The mRNAs encodi

SUBMITTER: Agca C 

PROVIDER: S-EPMC4048373 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature