Coregulation of transcription factor binding and nucleosome occupancy through DNA features of mammalian enhancers.

ABSTRACT: Transcription factors (TFs) preferentially bind sites contained in regions of computationally predicted high nucleosomal occupancy, suggesting that nucleosomes are gatekeepers of TF binding sites. However, because of their complexity mammalian genomes contain millions of randomly occurring, unbound TF consensus binding sites. We hypothesized that the information controlling nucleosome assembly may coincide with the information that enables TFs to bind cis-regulatory elements while ignoring randomly occurring sites. Hence, nucleosomes would selectively mask genomic sites that can be contacted by TFs and thus be potentially functional. The hematopoietic pioneer TF Pu.1 maintained nucleosome depletion at macrophage-specific enhancers that displayed a broad range of nucleosome occupancy in oth

SUBMITTER: Barozzi I 

PROVIDER: S-EPMC4048654 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature