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TRPV2 is critical for the maintenance of cardiac structure and function in mice.

ABSTRACT: The heart has a dynamic compensatory mechanism for haemodynamic stress. However, the molecular details of how mechanical forces are transduced in the heart are unclear. Here we show that the transient receptor potential, vanilloid family type 2 (TRPV2) cation channel is critical for the maintenance of cardiac structure and function. Within 4 days of eliminating TRPV2 from hearts of the adult mice, cardiac function declines severely, with disorganization of the intercalated discs that support mechanical coupling with neighbouring myocytes and myocardial conduction defects. After 9 days, cell shortening and Ca(2+) handling by single myocytes are impaired in TRPV2-deficient hearts. TRPV2-deficient neonatal cardiomyocytes form no intercalated discs and show no extracellular Ca(2+)-dependent intracellular Ca(2+) increase and insulin-like growth factor (IGF-1) secretion in response to stretch stimulation. We further demonstrate that IGF-1 receptor/PI3K/Akt pathway signalling is significantly downregulated in TRPV2-deficient hearts, and that IGF-1 administration partially prevents chamber dilation and impairment in cardiac pump function in these hearts. Our results improve our understanding of the molecular processes underlying the maintenance of cardiac structure and function.

SUBMITTER: Katanosaka Y

PROVIDER: S-EPMC4050274 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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TRPV2 is critical for the maintenance of cardiac structure and function in mice.

Katanosaka Yuki Y Iwasaki Keiichiro K Ujihara Yoshihiro Y Takatsu Satomi S Nishitsuji Koki K Kanagawa Motoi M Sudo Atsushi A Toda Tatsushi T Katanosaka Kimiaki K Mohri Satoshi S Naruse Keiji K

Nature communications 20140529

The heart has a dynamic compensatory mechanism for haemodynamic stress. However, the molecular details of how mechanical forces are transduced in the heart are unclear. Here we show that the transient receptor potential, vanilloid family type 2 (TRPV2) cation channel is critical for the maintenance of cardiac structure and function. Within 4 days of eliminating TRPV2 from hearts of the adult mice, cardiac function declines severely, with disorganization of the intercalated discs that support mec ...[more]

PMID: 24874017

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TRPV2 is critical for the maintenance of cardiac structure and function in mice.

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TRPV2 is critical for the maintenance of cardiac structure and function in mice.

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