Ontology highlight
ABSTRACT:
SUBMITTER: Huitema LF
PROVIDER: S-EPMC4050368 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Huitema Leonie F A LF Renn Jörg J Logister Ive I Gray Jerilyn K JK Waltz Susan E SE Flik Gert G Schulte-Merker Stefan S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20120711 10
To systematically identify novel gene functions essential for osteogenesis and skeletal mineralization, we performed a forward genetic mutagenesis screen in zebrafish and isolated a mutant that showed delayed skeletal mineralization. Analysis of the mutant phenotype in an osterix:nuclear-GFP transgenic background demonstrated that mutants contain osterix-expressing osteoblasts comparable to wild-type embryos. Positional cloning revealed a premature stop mutation in the macrophage-stimulating pro ...[more]