Circular permutation of a WW domain: folding still occurs after excising the turn of the folding-nucleating hairpin.
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ABSTRACT:
SUBMITTER: Kier BL
PROVIDER: S-EPMC4051316 | biostudies-literature | 2014 Jan
REPOSITORIES: biostudies-literature
Publications
Circular permutation of a WW domain: folding still occurs after excising the turn of the folding-nucleating hairpin.
Kier Brandon L BL Anderson Jordan M JM Andersen Niels H NH
Journal of the American Chemical Society 20140103 2
A hyperstable Pin1 WW domain has been circularly permuted via excision of the fold-nucleating turn; it still folds to form the native three-strand sheet and hydrophobic core features. Multiprobe folding dynamics studies of the normal and circularly permuted sequences, as well as their constituent hairpin fragments and comparable-length β-strand-loop-β-strand models, indicate 2-state folding for all topologies. N-terminal hairpin formation is the fold nucleating event for the wild-type sequence; ...[more]
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