Integrative gene transfer in the truffle Tuber borchii by Agrobacterium tumefaciens-mediated transformation.

ABSTRACT: Agrobacterium tumefaciens-mediated transformation is a powerful tool for reverse genetics and functional genomic analysis in a wide variety of plants and fungi. Tuber spp. are ecologically important and gastronomically prized fungi ("truffles") with a cryptic life cycle, a subterranean habitat and a symbiotic, but also facultative saprophytic lifestyle. The genome of a representative member of this group of fungi has recently been sequenced. However, because of their poor genetic tractability, including transformation, truffles have so far eluded in-depth functional genomic investigations. Here we report that A. tumefaciens can infect Tuber borchii mycelia, thereby conveying its transfer DNA with the production of stably integrated transformants. We constructed two new binary plasmids (pAB

SUBMITTER: Brenna A 

PROVIDER: S-EPMC4052689 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature