Ontology highlight
ABSTRACT:
SUBMITTER: Henard CA
PROVIDER: S-EPMC4053250 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Henard Calvin A CA Tapscott Timothy T Crawford Matthew A MA Husain Maroof M Doulias Paschalis-Thomas PT Porwollik Steffen S Liu Lin L McClelland Michael M Ischiropoulos Harry H Vázquez-Torres Andrés A
Molecular microbiology 20140107 4
We show that thiols in the 4-cysteine zinc-finger motif of DksA, an RNA polymerase accessory protein known to regulate the stringent response, sense oxidative and nitrosative stress. Hydrogen peroxide- or nitric oxide (NO)-mediated modifications of thiols in the DksA 4-cysteine zinc-finger motif release the metal cofactor and drive reversible changes in the α-helicity of the protein. Wild-type and relA spoT mutant Salmonella, but not isogenic dksA-deficient bacteria, experience the downregulatio ...[more]