Voom: Precision weights unlock linear model analysis tools for RNA-seq read counts.
Ontology highlight
ABSTRACT:
SUBMITTER: Law CW
PROVIDER: S-EPMC4053721 | biostudies-literature | 2014 Feb
REPOSITORIES: biostudies-literature
Publications
voom: Precision weights unlock linear model analysis tools for RNA-seq read counts.
Law Charity W CW Chen Yunshun Y Shi Wei W Smyth Gordon K GK
Genome biology 20140203 2
New normal linear modeling strategies are presented for analyzing read counts from RNA-seq experiments. The voom method estimates the mean-variance relationship of the log-counts, generates a precision weight for each observation and enters these into the limma empirical Bayes analysis pipeline. This opens access for RNA-seq analysts to a large body of methodology developed for microarrays. Simulation studies show that voom performs as well or better than count-based RNA-seq methods even when th ...[more]
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