Ontology highlight
ABSTRACT:
SUBMITTER: Dollive S
PROVIDER: S-EPMC4053730 | biostudies-literature | 2012 Jul
REPOSITORIES: biostudies-literature
Genome biology 20120703 7
Eukaryotic microorganisms are important but understudied components of the human microbiome. Here we present a pipeline for analysis of deep sequencing data on single cell eukaryotes. We designed a new 18S rRNA gene-specific PCR primer set and compared a published rRNA gene internal transcribed spacer (ITS) gene primer set. Amplicons were tested against 24 specimens from defined eukaryotes and eight well-characterized human stool samples. A software pipeline https://sourceforge.net/projects/broc ...[more]