Direct T cell-tumour interaction triggers TH1 phenotype activation through the modification of the mesenchymal stromal cells transcriptional programme.

ABSTRACT:

Background

Mesenchymal stromal cells (MSCs) are heterogeneous cells with immunoregulatory and wound-healing properties. In cancer, they are known to be an essential part of the tumour microenvironment. However, their role in tumour growth and rejection remains unclear. To investigate this, we co-cultured human MSCs, tumour infiltrating lymphocytes (TIL), and melanoma cells to investigate the role of MSCs in the tumour environment.

Methods

Mesenchymal stromal cells were co-cultured with melanoma antigen-specific TIL that were stimulated either with HLA-A*0201(+) melanoma cells or with a corresponding clone that had lost HLA-A*0201 expression.

Results

Activated TIL induced profound pro-inflammatory gene expression signature in MSCs. Analysis of culture supernatant found

SUBMITTER: Jin P 

PROVIDER: S-EPMC4056054 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature