FOCAL: an experimental design tool for systematizing metabolic discoveries and model development.
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ABSTRACT:
SUBMITTER: Tervo CJ
PROVIDER: S-EPMC4056367 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
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FOCAL: an experimental design tool for systematizing metabolic discoveries and model development.
Tervo Christopher J CJ Reed Jennifer L JL
Genome biology 20121213 12
Current computational tools can generate and improve genome-scale models based on existing data; however, for many organisms, the data needed to test and refine such models are not available. To facilitate model development, we created the forced coupling algorithm, FOCAL, to identify genetic and environmental conditions such that a reaction becomes essential for an experimentally measurable phenotype. This reaction's conditional essentiality can then be tested experimentally to evaluate whether ...[more]
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