Dataset Information

Dual-Emissive Difluoroboron Naphthyl-Phenyl ?-Diketonate Polylactide Materials: Effects of Heavy Atom Placement and Polymer Molecular Weight.

ABSTRACT: Luminescent materials are important for imaging and sensing. Aromatic difluoroboron ?-diketonate complexes (BF₂bdks) are classic fluorescent molecules that have been explored as photochemical reagents, two-photon dyes, and oxygen sensors. A series of BF₂bdks with naphthyl and phenyl groups was synthesized, and photophysical properties were investigated in both methylene chloride and poly(lactic acid) (PLA). Polymer molecular weight and dye attachment site along with bromide heavy atom placement were varied to tune optical properties of dye-PLA materials. Systems without heavy atoms have long phosphorescence lifetimes, which is useful for lifetime-based oxygen sensing. Bromine substitution on the naphthyl ring resulted in intense, clearly distinguishable fluorescence and phosphorescence peaks important for ratiometric oxygen sensing and imaging.

SUBMITTER: Samonina-Kosicka J

PROVIDER: S-EPMC4059218 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Publications

Dual-Emissive Difluoroboron Naphthyl-Phenyl β-Diketonate Polylactide Materials: Effects of Heavy Atom Placement and Polymer Molecular Weight.

Samonina-Kosicka Jelena J DeRosa Christopher A CA Morris William A WA Fan Ziyi Z Fraser Cassandra L CL

Macromolecules 20140523 11

Luminescent materials are important for imaging and sensing. Aromatic difluoroboron β-diketonate complexes (BF<sub>2</sub>bdks) are classic fluorescent molecules that have been explored as photochemical reagents, two-photon dyes, and oxygen sensors. A series of BF<sub>2</sub>bdks with naphthyl and phenyl groups was synthesized, and photophysical properties were investigated in both methylene chloride and poly(lactic acid) (PLA). Polymer molecular weight and dye attachment site along with bromide ...[more]

PMID: 24954954

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Project description:Critical for metabolism, oxygen plays an essential role in maintaining the structure and function of neurons. Oxygen sensing is important in common neurological disorders such as strokes, seizures, or neonatal hypoxic-ischemic injuries, which result from an imbalance between metabolic demand and oxygen supply. Phosphorescence quenching by oxygen provides a non-invasive optical method to measure oxygen levels within cells and tissues. Difluoroboron ?-diketonates are a family of luminophores with high quantum yields and tunable fluorescence and phosphorescence when embedded in certain rigid matrices such as poly (lactic acid) (PLA). Boron nanoparticles (BNPs) can be fabricated from dye-PLA materials for oxygen mapping in a variety of biological milieu. These dual-emissive nanoparticles have oxygen-insensitive fluorescence, oxygen-sensitive phosphorescence, and rigid matrix all in one, enabling real-time ratiometric oxygen sensing at micron-level spatial and millisecond-level temporal resolution. In this study, BNPs are applied in mouse brain slices to investigate oxygen distributions and neuronal activity. The optical properties and physical stability of BNPs in a biologically relevant buffer were stable. Primary neuronal cultures were labeled by BNPs and the mitochondria membrane probe MitoTracker Red FM. BNPs were taken up by neuronal cell bodies, at dendrites, and at synapses, and the localization of BNPs was consistent with that of MitoTracker Red FM. The brain slices were stained with the BNPs, and the BNPs did not significantly affect the electrophysiological properties of neurons. Oxygen maps were generated in living brain slices where oxygen is found to be mostly consumed by mitochondria near synapses. Finally, the BNPs exhibited excellent response when the conditions varied from normoxic to hypoxic and when the neuronal activity was increased by increasing K+ concentration. This work demonstrates the capability of BNPs as a non-invasive tool in oxygen sensing and could provide fundamental insight into neuronal mechanisms and excitability research.

Dataset Information

Dual-Emissive Difluoroboron Naphthyl-Phenyl ?-Diketonate Polylactide Materials: Effects of Heavy Atom Placement and Polymer Molecular Weight.

Publications

Dual-Emissive Difluoroboron Naphthyl-Phenyl β-Diketonate Polylactide Materials: Effects of Heavy Atom Placement and Polymer Molecular Weight.

Similar Datasets

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