Dataset Information

CD4?CD25?/highCD127low/? regulatory T cells are enriched in rheumatoid arthritis and osteoarthritis joints--analysis of frequency and phenotype in synovial membrane, synovial fluid and peripheral blood.

ABSTRACT:

Introduction

CD4?CD25?/highCD127low/? regulatory T cells (Tregs) play a crucial role in maintaining peripheral tolerance. Data about the frequency of Tregs in rheumatoid arthritis (RA) are contradictory and based on the analysis of peripheral blood (PB) and synovial fluid (SF). Because Tregs exert their anti-inflammatory activity in a contact-dependent manner, the analysis of synovial membrane (SM) is crucial. Published reports regarding this matter are lacking, so we investigated the distribution and phenotype of Tregs in concurrent samples of SM, SF and PB of RA patients in comparison to those of osteoarthritis (OA) patients.

Methods

Treg frequency in a total of 40 patients (18 RA and 22 OA) matched for age and sex was assessed by flow cytometry. Functional status was assessed by analysis of cell surface markers representative of activation, memory and regulation.

Results

CD4? T cells infiltrate the SM to higher frequencies in RA joints than in OA joints (P?=?0.0336). In both groups, Tregs accumulate more within the SF and SM than concurrently in PB (P?ConclusionsTreg enrichment into the joint compartment is not specific to inflammatory arthritis, as we found that it was similarly enriched in OA. RA pathophysiology might not be due to a Treg deficiency, because Treg concentration in SM was significantly higher in RA. Synovial Tregs represent a distinct phenotype and are activated effector memory cells (CD62L?CD69?), whereas peripheral Tregs are resting central memory cells (CD62L?CD69?).

SUBMITTER: Moradi B

PROVIDER: S-EPMC4060198 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature

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CD4⁺CD25⁺/highCD127low/⁻ regulatory T cells are enriched in rheumatoid arthritis and osteoarthritis joints--analysis of frequency and phenotype in synovial membrane, synovial fluid and peripheral blood.

Moradi Babak B Schnatzer Philipp P Hagmann Sébastien S Rosshirt Nils N Gotterbarm Tobias T Kretzer Jan Philippe JP Thomsen Marc M Lorenz Hanns-Martin HM Zeifang Felix F Tretter Theresa T

Arthritis research & therapy 20140417 2

<h4>Introduction</h4>CD4⁺CD25⁺/highCD127low/⁻ regulatory T cells (Tregs) play a crucial role in maintaining peripheral tolerance. Data about the frequency of Tregs in rheumatoid arthritis (RA) are contradictory and based on the analysis of peripheral blood (PB) and synovial fluid (SF). Because Tregs exert their anti-inflammatory activity in a contact-dependent manner, the analysis of synovial membrane (SM) is crucial. Published reports regarding this matter are lacking, so we investigated the di ...[more]

PMID: 24742142

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