Membrane raft association is a determinant of plasma membrane localization.

ABSTRACT: The lipid raft hypothesis proposes lateral domains driven by preferential interactions between sterols, sphingolipids, and specific proteins as a central mechanism for the regulation of membrane structure and function; however, experimental limitations in defining raft composition and properties have prevented unequivocal demonstration of their functional relevance. Here, we establish a quantitative, functional relationship between raft association and subcellular protein sorting. By systematic mutation of the transmembrane and juxtamembrane domains of a model transmembrane protein, linker for activation of T-cells (LAT), we generated a panel of variants possessing a range of raft affinities. These mutations revealed palmitoylation, transmembrane domain length, and transmembrane sequence t

SUBMITTER: Diaz-Rohrer BB 

PROVIDER: S-EPMC4060687 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature