Ontology highlight
ABSTRACT:
SUBMITTER: Reichman S
PROVIDER: S-EPMC4060726 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Reichman Sacha S Terray Angélique A Slembrouck Amélie A Nanteau Céline C Orieux Gaël G Habeler Walter W Nandrot Emeline F EF Sahel José-Alain JA Monville Christelle C Goureau Olivier O
Proceedings of the National Academy of Sciences of the United States of America 20140527 23
Progress in retinal-cell therapy derived from human pluripotent stem cells currently faces technical challenges that require the development of easy and standardized protocols. Here, we developed a simple retinal differentiation method, based on confluent human induced pluripotent stem cells (hiPSC), bypassing embryoid body formation and the use of exogenous molecules, coating, or Matrigel. In 2 wk, we generated both retinal pigmented epithelial cells and self-forming neural retina (NR)-like str ...[more]