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Isolation of single-base genome-edited human iPS cells without antibiotic selection.

ABSTRACT: Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells.

SUBMITTER: Miyaoka Y

PROVIDER: S-EPMC4063274 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Isolation of single-base genome-edited human iPS cells without antibiotic selection.

Miyaoka Yuichiro Y Chan Amanda H AH Judge Luke M LM Yoo Jennie J Huang Miller M Nguyen Trieu D TD Lizarraga Paweena P PP So Po-Lin PL Conklin Bruce R BR

Nature methods 20140209 3

Precise editing of human genomes in pluripotent stem cells by homology-driven repair of targeted nuclease-induced cleavage has been hindered by the difficulty of isolating rare clones. We developed an efficient method to capture rare mutational events, enabling isolation of mutant lines with single-base substitutions without antibiotic selection. This method facilitates efficient induction or reversion of mutations associated with human disease in isogenic human induced pluripotent stem cells. ...[more]

PMID: 24509632

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