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Cycle length and COD/N ratio determine properties of aerobic granules treating high-nitrogen wastewater.

ABSTRACT: Aerobic granule characteristic in sequencing batch reactors treating high-nitrogen digester supernatant was investigated at cycle lengths (t) of 6, 8 and 12 h with the COD/N ratios in the influent of 4.5 and 2.3. The biomass production (Y obs) correlated with the extracellular polymeric substances (EPS) in grams per COD removed. Denitrification efficiency significantly decreased as the amount of EPS in biomass increased, suggesting that organic assimilation in EPS hampers nitrogen removal. Granule hydrophobicity was highest at t of 8 h; the t has to be long enough to remove pollutants, but not so long that excessive biomass starvation causes extracellular protein consumption that decreases hydrophobicity. At a given t, reducing the COD/N ratio improved hydrophobicity that stimulates cell aggregation. At t of 6 h and the COD/N ratio of 2.3, the dominance of 0.5-1.0 mm granules favored simultaneous nitrification and denitrification and resulted in the highest nitrogen removal.

SUBMITTER: Cydzik-Kwiatkowska A

PROVIDER: S-EPMC4065379 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Cycle length and COD/N ratio determine properties of aerobic granules treating high-nitrogen wastewater.

Cydzik-Kwiatkowska Agnieszka A Bernat Katarzyna K Zielińska Magdalena M Wojnowska-Baryła Irena I

Bioprocess and biosystems engineering 20131210 7

Aerobic granule characteristic in sequencing batch reactors treating high-nitrogen digester supernatant was investigated at cycle lengths (t) of 6, 8 and 12 h with the COD/N ratios in the influent of 4.5 and 2.3. The biomass production (Y obs) correlated with the extracellular polymeric substances (EPS) in grams per COD removed. Denitrification efficiency significantly decreased as the amount of EPS in biomass increased, suggesting that organic assimilation in EPS hampers nitrogen removal. Granu ...[more]

PMID: 24317485

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Project description:Aerobic granular sludge is attractive for high-rate biological wastewater treatment. Biomass wash-out conditions stimulate the formation of aerobic granules. Deteriorated performances in biomass settling and nutrient removal during start-up have however often been reported. The effect of wash-out dynamics was investigated on bacterial selection, biomass settling behavior, and metabolic activities during the formation of early-stage granules from activated sludge of two wastewater treatment plants (WWTP) over start-up periods of maximum 60 days. Five bubble-column sequencing batch reactors were operated with feast-famine regimes consisting of rapid pulse or slow anaerobic feeding followed by aerobic starvation. Slow-settling fluffy granules were formed when an insufficient superficial air velocity (SAV; 1.8 cm s(-1)) was applied, when the inoculation sludge was taken from a WWTP removing organic matter only, or when reactors were operated at 30°C. Fast-settling dense granules were obtained with 4.0 cm s(-1) SAV, or when the inoculation sludge was taken from a WWTP removing all nutrients biologically. However, only carbon was aerobically removed during start-up. Fluffy granules and dense granules were displaying distinct predominant phylotypes, namely filamentous Burkholderiales affiliates and Zoogloea relatives, respectively. The latter were predominant in dense granules independently from the feeding regime. A combination of insufficient solid retention time and of leakage of acetate into the aeration phase during intensive biomass wash-out was the cause for the proliferation of Zoogloea spp. in dense granules, and for the deterioration of BNR performances. It is however not certain that Zoogloea-like organisms are essential in granule formation. Optimal operation conditions should be elucidated for maintaining a balance between organisms with granulation propensity and nutrient removing organisms in order to form granules with BNR activities in short start-up periods.

Project description:Simultaneous nitrification-denitrification (SND) is, in theory, a key advantage of aerobic granular sludge systems over conventional activated sludge systems. But practical experience and literature suggests that SND and thus total nitrogen removal are limited during treatment of municipal wastewater using AGS systems. This study thus aims at quantifying the extent and understanding the mechanisms of SND during treatment of municipal wastewater with aerobic granular sludge (AGS) systems. Experiments (long-term and batch-tests) as well as mathematical modelling were performed. Our experimental results demonstrate that SND is significantly limited during treatment of low-strength municipal wastewater with AGS systems (14-39%), while almost full SND is observed when treating synthetic influent containing only diffusible substrate (90%). Our simulations demonstrate that the main mechanisms behind limited SND are (1) the dynamics of anoxic zone formation inside the granule, (2) the diffusibility and availability of electron-donors in those zones and (3) the aeration mode. The development of anoxic zones is driven by the utilisation of oxygen in the upper layers of the granule leading to transport limitations of oxygen inside the granule; this effect is closely linked to granule size and wastewater composition. Development of anoxic zones during the aerobic phase is limited for small granules at constant aeration at bulk dissolved oxygen (DO) concentration of 2 mgO2 L-1, and anoxic zones only develop during a brief period of the aerated phase for large granules. Modelling results further indicate that a large fraction of electron-donors are actually utilised in aerobic rather than anoxic redox zones - in the bulk or at the granule surface. Thus, full SND cannot be achieved with AGS treating low strength municipal wastewater if a constant DO is maintained during the aeration phase. Optimised aeration strategies are therefore required. 2-step and alternating aeration are tested successfully using mathematical modelling and increase TN removal to 40-79%, without compromising nitrification, and by shifting electron-donor utilisation towards anoxic redox conditions.

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Cycle length and COD/N ratio determine properties of aerobic granules treating high-nitrogen wastewater.

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Cycle length and COD/N ratio determine properties of aerobic granules treating high-nitrogen wastewater.

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