Binding affinity and cooperativity control U2B?/snRNA/U2A' RNP formation.
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ABSTRACT:
SUBMITTER: Williams SG
PROVIDER: S-EPMC4067145 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Publications
Binding affinity and cooperativity control U2B″/snRNA/U2A' RNP formation.
Williams Sandra G SG Hall Kathleen B KB
Biochemistry 20140605 23
The U1A and U2B″ proteins are components of the U1 and U2 snRNPs, respectively, where they bind to snRNA stemloops. While localization of U1A and U2B″ to their respective snRNP is a well-known phenomenon, binding of U2B″ to U2 snRNA is typically thought to be accompanied by the U2A' protein. The molecular mechanisms that lead to formation of the RNA/U2B″/U2A' complex and its localization to the U2 snRNP are investigated here, using a combination of in vitro RNA-protein and protein-protein fluore ...[more]
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