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Radiation Damage to DNA: The Indirect Effect of Low Energy Electrons.

ABSTRACT: We report the effect of DNA hydration level on damage yields induced by soft X-rays and photo-emitted low energy electrons (LEEs) in thin films of plasmid DNA irradiated in N2 at atmospheric pressure under different humidity levels. Contrary to a dilute solution of DNA, the number of H2O molecules per nucleotide (?) in these films can be varied from ?=2.5 to ~33, where ??20 corresponds to layers of hydration and ?=33 to an additional bulk-like water layer. Our results indicate that DNA damage induced by LEEs does not increase significantly until the second hydration shell is formed. However, this damage increases dramatically as DNA coverage approaches bulk-like hydration conditions. A number of phenomena are invoked to account for these behaviors including: dissociative electron transfer from water-interface electron traps to DNA bases, quenching of dissociative electron attachment to DNA and quenching of dissociative electronically excited states of H2O in contact with DNA.

SUBMITTER: Alizadeh E

PROVIDER: S-EPMC4071054 | biostudies-literature | 2013 Feb

REPOSITORIES: biostudies-literature

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Radiation Damage to DNA: The Indirect Effect of Low Energy Electrons.

Alizadeh Elahe E Sanz Ana G AG García Gustavo G Sanche Léon L

The journal of physical chemistry letters 20130201 5

We report the effect of DNA hydration level on damage yields induced by soft X-rays and photo-emitted low energy electrons (LEEs) in thin films of plasmid DNA irradiated in N<sub>2</sub> at atmospheric pressure under different humidity levels. Contrary to a dilute solution of DNA, the number of H<sub>2</sub>O molecules per nucleotide (Γ) in these films can be varied from Γ=2.5 to ~33, where Γ≤20 corresponds to layers of hydration and Γ=33 to an additional bulk-like water layer. Our results indic ...[more]

PMID: 24976899

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Project description:Irradiation induced damage in materials is highly detrimental and is a critical issue in several vital science and technology fields, e.g., the nuclear and space industries. While the effect of dimensionality (nano/bulk) of materials on its radiation damage tolerance has been receiving tremendous interest, studies have only concentrated on low energy (nuclear energy loss (Sn) dominant) and high energy (electronic energy loss (Se) dominant) irradiations independently (wherein, interestingly, the effect is opposite). In-fact, research on radiation damage in general has almost entirely focused only on independent irradiations with low and/or high energy particles till date, and investigations under simultaneous impingement of energetic particles (which also correspond to the actual irradiation conditions during real-world applications) are very scarce. The present work elucidates, taking cubic zirconia as a model system, the effect of grain size (26 nm vs 80 nm) on the radiation tolerance against simultaneous irradiation with low energy (900 keV I) and high energy (27 meV Fe) particles/ions; and, in particular, introduces the enhancement in the radiation damage tolerance upon downsizing from bulk to nano dimension. This result is interpreted within the framework of the thermal-spike model after considering (1) the fact that there is essentially no spatial and time overlap between the damage events of the two 'simultaneous' irradiations, and (2) the influence of grain size on radiation damage against individual Sn and Se. The present work besides providing the first fundamental insights into how the grain size/grain boundary density inherently mediates the radiation response of a material to simultaneous Sn and Se deposition, also (1) paves the way for potential application of nano-crystalline materials in the nuclear industry (where simultaneous irradiations with low and high energy particles correspond to the actual irradiation conditions), and (2) lays the groundwork for understanding the material behaviour under other simultaneous (viz. Sn and Sn, Se and Se) irradiations.

Dataset Information

Radiation Damage to DNA: The Indirect Effect of Low Energy Electrons.

Publications

Radiation Damage to DNA: The Indirect Effect of Low Energy Electrons.

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