Unknown

Dataset Information

0

Overexpression of D-xylose reductase (xyl1) gene and antisense inhibition of D-xylulokinase (xyiH) gene increase xylitol production in Trichoderma reesei.


ABSTRACT: T. reesei is an efficient cellulase producer and biomass degrader. To improve xylitol production in Trichoderma reesei strains by genetic engineering, two approaches were used in this study. First, the presumptive D-xylulokinase gene in T. reesei (xyiH), which has high homology to known fungi D-xylulokinase genes, was silenced by transformation of T. reesei QM9414 strain with an antisense construct to create strain S6-2-2. The expression of the xyiH gene in the transformed strain S6-2-2 decreased at the mRNA level, and D-xylulokinase activity decreased after 48?h of incubation. This led to an increase in xylitol production from undetectable levels in wild-type T. reesei QM9414 to 8.6?mM in S6-2-2. The T. reesei ?xdh is a xylose dehydrogenase knockout strain with increased xylitol production compared to the wild-type T. reesei QM9414 (22.8?mM versus undetectable). The copy number of the xylose reductase gene (xyl1) in T. reesei ?xdh strain was increased by genetic engineering to create a new strain ?9-5-1. The ?9-5-1 strain showed a higher xyl1 expression and a higher yield of xylose reductase, and xylitol production was increased from 22.8?mM to 24.8?mM. Two novel strains S6-2-2 and ?9-5-1 are capable of producing higher yields of xylitol. T. reesei has great potential in the industrial production of xylitol.

SUBMITTER: Hong Y 

PROVIDER: S-EPMC4071787 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

altmetric image

Publications

Overexpression of D-xylose reductase (xyl1) gene and antisense inhibition of D-xylulokinase (xyiH) gene increase xylitol production in Trichoderma reesei.

Hong Yuanyuan Y   Dashtban Mehdi M   Kepka Greg G   Chen Sanfeng S   Qin Wensheng W  

BioMed research international 20140611


T. reesei is an efficient cellulase producer and biomass degrader. To improve xylitol production in Trichoderma reesei strains by genetic engineering, two approaches were used in this study. First, the presumptive D-xylulokinase gene in T. reesei (xyiH), which has high homology to known fungi D-xylulokinase genes, was silenced by transformation of T. reesei QM9414 strain with an antisense construct to create strain S6-2-2. The expression of the xyiH gene in the transformed strain S6-2-2 decrease  ...[more]

Similar Datasets

| S-EPMC93154 | biostudies-literature
| S-EPMC3250611 | biostudies-literature
| S-EPMC5925849 | biostudies-literature
| S-EPMC3699558 | biostudies-literature
| S-EPMC3953602 | biostudies-literature
| S-EPMC201247 | biostudies-literature
| S-EPMC1489653 | biostudies-literature
| S-EPMC3623455 | biostudies-literature
| S-EPMC4955148 | biostudies-literature
| S-EPMC3334502 | biostudies-literature