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A biocatalytic cascade with several output signals--towards biosensors with different levels of confidence.

ABSTRACT: The biocatalytic cascade based on enzyme-catalyzed reactions activated by several biomolecular input signals and producing output signal after each reaction step was developed as an example of a logically reversible information processing system. The model system was designed to mimic the operation of concatenated AND logic gates with optically readable output signals generated at each step of the logic operation. Implications include concurrent bioanalyses and data interpretation for medical diagnostics.

SUBMITTER: Guz N

PROVIDER: S-EPMC4072206 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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A biocatalytic cascade with several output signals--towards biosensors with different levels of confidence.

Guz Nataliia N Halámek Jan J Rusling James F JF Katz Evgeny E

Analytical and bioanalytical chemistry 20140420 14

The biocatalytic cascade based on enzyme-catalyzed reactions activated by several biomolecular input signals and producing output signal after each reaction step was developed as an example of a logically reversible information processing system. The model system was designed to mimic the operation of concatenated AND logic gates with optically readable output signals generated at each step of the logic operation. Implications include concurrent bioanalyses and data interpretation for medical di ...[more]

PMID: 24748446

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