Unknown

Dataset Information

0

Mapping-by-sequencing accelerates forward genetics in barley.


ABSTRACT: Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene, which is confirmed by the analysis of independent mutant alleles. Our method illustrates how the genomic resources of barley together with exome resequencing can underpin mapping-by-sequencing.

SUBMITTER: Mascher M 

PROVIDER: S-EPMC4073093 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Mapping-by-sequencing accelerates forward genetics in barley.

Mascher Martin M   Jost Matthias M   Kuon Joel-Elias JE   Himmelbach Axel A   Aßfalg Axel A   Beier Sebastian S   Scholz Uwe U   Graner Andreas A   Stein Nils N  

Genome biology 20140610 6


Mapping-by-sequencing has emerged as a powerful technique for genetic mapping in several plant and animal species. As this resequencing-based method requires a reference genome, its application to complex plant genomes with incomplete and fragmented sequence resources remains challenging. We perform exome sequencing of phenotypic bulks of a mapping population of barley segregating for a mutant phenotype that increases the rate of leaf initiation. Read depth analysis identifies a candidate gene,  ...[more]

Similar Datasets

| S-EPMC9284286 | biostudies-literature
| S-EPMC5295590 | biostudies-literature
2010-12-31 | E-GEOD-24511 | biostudies-arrayexpress
2010-12-31 | GSE24511 | GEO
| S-EPMC7883666 | biostudies-literature
2011-08-02 | GSE31038 | GEO
2011-11-22 | GSE33394 | GEO
2011-08-01 | E-GEOD-31038 | biostudies-arrayexpress
| S-EPMC5839405 | biostudies-literature
2010-09-12 | E-GEOD-22024 | biostudies-arrayexpress