Ontology highlight
ABSTRACT:
SUBMITTER: Okuyama MW
PROVIDER: S-EPMC4073351 | biostudies-literature | 2014 May
REPOSITORIES: biostudies-literature
Okuyama Minami W MW Shimozuru Michito M Tsubota Toshio T
The Journal of veterinary medical science 20140123 5
A genetic method for sex determination in raccoons was developed based on nucleotide differences of the zinc finger protein genes ZFX and ZFY. Four novel internal primers specific for ZFX or ZFY were designed. PCR amplification using two primer sets followed by agarose gel electrophoresis enabled sex determination. 141-bp and 447-bp bands were in both sex, and 346-bp band was specific only in male with primer set I. 345-bp and 447-bp bands were in both sex, and 141-bp band was specific only in m ...[more]