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Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis.

ABSTRACT: To assess the variability of follicular fluid (FF) high-density lipoprotein (HDL) particle components.Cross-sectional design with assessment using two-stage nested analysis of variance.Reproductive health center.180 in vitro fertilization (IVF) patients.None.Nineteen HDL components including HDL cholesterol and free (unesterified) and esterified forms, phospholipids, triglycerides, apolipoproteins A-1 and A-2, paraoxonase 1 (PON1) activities, and seven lipophilic vitamins and micronutrients.We collected FF specimens from two contralateral follicles on the day of oocyte retrieval and analyzed them for HDL components, characterizing the analytes by age, body mass index (BMI), race and smoking. For some analytes, a majority of total measurement variability was attributed to sources between follicles, suggesting an important role for the integrity of the blood-follicle barrier and in situ remodeling of plasma-derived constituents. For other analytes, variability was mostly attributed to sources between women, likely indicative of plasma levels. Variability between follicles decreased with increasing age and differed by BMI and smoking, and generally were lower for Asians and women with diminished ovarian reserve.Substantial variability in FF HDL components exist between follicles among women undergoing IVF as well as between women by age, BMI, race, smoking, and infertility diagnosis.

SUBMITTER: Bloom MS

PROVIDER: S-EPMC4077611 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

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Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis.

Bloom Michael S MS Kim Keewan K Fujimoto Victor Y VY Browne Richard W RW

Fertility and sterility 20140226 5

<h4>Objective</h4>To assess the variability of follicular fluid (FF) high-density lipoprotein (HDL) particle components.<h4>Design</h4>Cross-sectional design with assessment using two-stage nested analysis of variance.<h4>Setting</h4>Reproductive health center.<h4>Patient(s)</h4>180 in vitro fertilization (IVF) patients.<h4>Intervention(s)</h4>None.<h4>Main outcome measure(s)</h4>Nineteen HDL components including HDL cholesterol and free (unesterified) and esterified forms, phospholipids, trigly ...[more]

PMID: 24581578

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Project description:Study questionDo charged iron particles, components of space radiation, cause premature ovarian failure?Summary answerExposure to charged iron particles causes ovarian DNA damage, oxidative damage and apoptosis, resulting in premature ovarian failure.What is known alreadyThe ovary is very sensitive to follicle destruction by low linear energy transfer (LET) radiation, such as X-rays and γ-rays. However, it is completely unknown whether high-LET radiation, such as charged iron particles, also destroys ovarian follicles.Study design, size, durationTwelve week old C57BL/6J female mice were exposed to single doses of 0, 5, 30 or 50 cGy (n = 8/group) charged iron particles (LET = 179 keV/µm) at energy of 600 MeV/u. Two groups were irradiated at the highest dose, one fed AIN-93M chow and the other fed AIN-93M chow supplemented with 150 mg/kg diet alpha lipoic acid (ALA).Participants/materials, setting, methodsWe quantified the numbers of ovarian follicles, measured serum follicle stimulating hormone (FSH) and luteinizing hormone (LH) concentrations, and analyzed histone H2AX phosphorylation, oxidative damage and apoptosis markers in the ovarian follicles.Main results and the role of chanceH2AX phosphorylation, lipid peroxidation, protein nitration and apoptosis were highly induced in ovarian follicles at 6 h and remained increased 1 week after irradiation. As a result, numbers of healthy ovarian follicles were significantly and dose-dependently depleted at 1 and 8 weeks post-irradiation, with 57, 84 and 99% decreases in primordial follicles at 8 weeks at the 5, 30 and 50 cGy doses, respectively (P < 0.05 versus 0 cGy). Consistent with near-total depletion of ovarian follicles in the 50 cGy group, serum concentrations of FSH and LH were significantly elevated at 8 weeks. Dietary supplementation with ALA partially prevented the adverse ovarian effects of 50 cGy iron particles.Limitations, reasons for cautionAbout 21% of the estimated radiation dose from exposure to galactic cosmic rays during a multi-year Mars mission will be due to high-LET particles, of which iron is only one. The effects of galactic cosmic rays, which contain a mixture of multiple charged particles, as well as protons, neutrons, and helium ions, may differ from the effects of iron alone.Wider implications of the findingsWe show for the first time that charged high-LET ions are highly damaging to the ovary even at low doses, causing premature ovarian failure. In addition to raising concerns for female astronauts, these findings raise concerns for ovarian damage due to clinical uses of high-LET particles for cancer treatment. In addition to causing infertility, premature ovarian failure has adverse implications for the functions of heart, brain, bone and muscle later in life.Study funding/competing interestsThis work was supported by a National Aeronautics and Space Administration grant NNX14AC50G to U.L. B.M. was partially supported by a National Space Biomedical Research Institute First Award, PF04302. Additional support was received from the University of California Irvine Center for Occupational and Environmental Health. The authors have no conflicts of interests.

Project description:Objective- The cell-cholesterol efflux capacity of HDL (high-density lipoprotein) is inversely associated with coronary heart disease risk. ABCA1 (ATP-binding cassette transporter A1) plays a crucial role in cholesterol efflux from macrophages to pre?-1-HDL. We tested the hypothesis that coronary heart disease patients have functionally abnormal pre?-1-HDL. Approach and Results- HDL cell-cholesterol efflux capacity via the ABCA1 and the SR-BI (scavenger receptor class B type I) pathways, HDL antioxidative capacity, apo (apolipoprotein) A-I-containing HDL particles, and inflammatory- and oxidative-stress markers were measured in a case-control study of 100 coronary heart disease cases and 100 sex-matched controls. There were significant positive correlations between ABCA1-dependent cholesterol efflux and the levels of small lipid-poor pre?-1 particles ( R2=0.535) and between SR-BI-dependent cholesterol efflux and the levels of large lipid-rich (?-1+?-2) HDL particles ( R2=0.712). Cases had significantly higher (87%) pre?-1 concentrations than controls, but the functionality of their pre?-1 particles (pre?-1 concentration normalized ABCA1-dependent efflux capacity) was significantly lower (-31%). Cases had significantly lower (-12%) mean concentration of large HDL particles, but the functionality of their particles (?-1+?-2 concentration normalized SR-BI-dependent efflux capacity) was significantly higher (22%) compared with that of controls. HDL antioxidative capacity was significantly lower (-16%) in cases than in controls. There were no significant correlations between either pre?-1 functionality or large HDL particle functionality with HDL antioxidative capacity or the concentrations of inflammatory- and oxidative-stress markers. Conclusions- HDL cell-cholesterol efflux capacity is significantly influenced by both the concentration and the functionality of specific HDL particles participating in cell-cholesterol efflux. Coronary heart disease patients have higher than normal pre?-1 concentrations with decreased functionality and lower than normal large HDL particle concentrations with enhanced functionality.

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Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis.

Publications

Variability in the components of high-density lipoprotein particles measured in human ovarian follicular fluid: a cross-sectional analysis.

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