Dataset Information

Th17/Treg ratio derived using DNA methylation analysis is associated with the late phase asthmatic response.

ABSTRACT:

Background

The imbalance between Th17 and Treg cells has been studied in various diseases including allergic asthma but their roles have not been fully understood in the development of the late phase asthmatic response.

Objectives

To determine changes in Th17 and Treg cell numbers between isolated early responders (ERs) and dual responders (DRs) undergoing allergen inhalation challenge. To identify gene expression profiles associated with Th17 and Treg cells.

Methods

14 participants (8 ERs and 6 DRs) with mild allergic asthma underwent allergen inhalation challenge. Peripheral blood was collected prior to and 2 hours post allergen challenge. DNA methylation analysis was used to quantifiy the relative frequencies of Th17, Tregs, total B cells, and total T cells. Gene expression from whole blood was measured using microarrays. Technical replication of selected genes was performed using nanoString nCounter Elements.

Results

The Th17/Treg ratio significantly increased in DRs compared to ERs post allergen challenge compared to pre-challenge. Genes significantly correlated to Th17 and Treg cell counts were inversely correlated with each other. Genes significantly correlated with Th17/Treg ratio included the cluster of genes of the leukocyte receptor complex located on chromosome 19q 13.4.

Conclusions

Th17/Treg imbalance post-challenge may contribute to the development of the late phase inflammatory phenotype.

SUBMITTER: Singh A

PROVIDER: S-EPMC4078401 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Publications

Th17/Treg ratio derived using DNA methylation analysis is associated with the late phase asthmatic response.

Singh Amrit A Yamamoto Masatsugu M Ruan Jian J Choi Jung Young JY Gauvreau Gail M GM Olek Sven S Hoffmueller Ulrich U Carlsten Christopher C FitzGerald J Mark JM Boulet Louis-Philippe LP O'Byrne Paul M PM Tebbutt Scott J SJ

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology 20140624 1

<h4>Background</h4>The imbalance between Th17 and Treg cells has been studied in various diseases including allergic asthma but their roles have not been fully understood in the development of the late phase asthmatic response.<h4>Objectives</h4>To determine changes in Th17 and Treg cell numbers between isolated early responders (ERs) and dual responders (DRs) undergoing allergen inhalation challenge. To identify gene expression profiles associated with Th17 and Treg cells.<h4>Methods</h4>14 par ...[more]

PMID: 24991220

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Dataset Information

Th17/Treg ratio derived using DNA methylation analysis is associated with the late phase asthmatic response.

Background

Objectives

Methods

Results

Conclusions

Publications

Th17/Treg ratio derived using DNA methylation analysis is associated with the late phase asthmatic response.

Similar Datasets

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