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Marker for type VI secretion system effectors.

ABSTRACT: Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic bacterial proteins that are primarily located in the T6SS genome neighborhood. We demonstrate that several MIX-containing proteins are T6SS effectors and that they are not required for T6SS activity. Thus, we propose that MIX-containing proteins are T6SS effectors. Our findings allow for the identification of numerous uncharacterized T6SS effectors that will undoubtedly lead to the discovery of new biological mechanisms.

SUBMITTER: Salomon D 

PROVIDER: S-EPMC4078801 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature

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Marker for type VI secretion system effectors.

Salomon Dor D   Kinch Lisa N LN   Trudgian David C DC   Guo Xiaofeng X   Klimko John A JA   Grishin Nick V NV   Mirzaei Hamid H   Orth Kim K  

Proceedings of the National Academy of Sciences of the United States of America 20140609 25

Bacteria use diverse mechanisms to kill, manipulate, and compete with other cells. The recently discovered type VI secretion system (T6SS) is widespread in bacterial pathogens and used to deliver virulence effector proteins into target cells. Using comparative proteomics, we identified two previously unidentified T6SS effectors that contained a conserved motif. Bioinformatic analyses revealed that this N-terminal motif, named MIX (marker for type six effectors), is found in numerous polymorphic  ...[more]