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Targeted highly sensitive detection/eradication of multi-drug resistant Salmonella DT104 through gold nanoparticle-SWCNT bioconjugated nanohybrids.

ABSTRACT: Monoclonal antibody-conjugated sphere-shaped gold nanoparticles were combined with single-walled carbon nanotubes (SWCNTs) to create a nanohybrid system to selectively detect and eradicate multiple drug resistant Salmonella (MDRS) typhimurium DT104 bacteria. The Raman signal intensity from Rhodamine 6G (Rh6G) modified monoclonal AC04 antibody SWCNTs-gold nanoparticle (SWCNT-GNPs) hybrid provided a SERS enhancement by several orders of magnitude to detect the MDRS bacteria over the GNP system. A targeted photothermolysis experiment using 670 nm light at 2 W cm(-2) for 15 min, resulted in selective and irreparable damage to more than 99% Salmonella DT104 at the concentration of 10(5) CFU mL(-1). In comparison to solely SWCNTs or GNPs, our SWCNT-GNPs nanohybrids have also shown a better photothermal efficiency.

SUBMITTER: Lin Y

PROVIDER: S-EPMC4078871 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature

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Targeted highly sensitive detection/eradication of multi-drug resistant Salmonella DT104 through gold nanoparticle-SWCNT bioconjugated nanohybrids.

Lin Yunfeng Y Hamme Ii Ashton T AT

The Analyst 20140801 15

Monoclonal antibody-conjugated sphere-shaped gold nanoparticles were combined with single-walled carbon nanotubes (SWCNTs) to create a nanohybrid system to selectively detect and eradicate multiple drug resistant Salmonella (MDRS) typhimurium DT104 bacteria. The Raman signal intensity from Rhodamine 6G (Rh6G) modified monoclonal AC04 antibody SWCNTs-gold nanoparticle (SWCNT-GNPs) hybrid provided a SERS enhancement by several orders of magnitude to detect the MDRS bacteria over the GNP system. A ...[more]

PMID: 24897935

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Project description:In this work, multifunctional nanocarriers consisting of poly(sodium-4-styrenesulfonate) (PSS)/doxorubicin (DOXO)/poly-l-lysine hydrobromide (PLL)/hyaluronic acid (HA)-coated and (PSS/DOXO/PLL)2/HA-coated gold nanorods were assembled by the layer-by-layer technique with the aims of coupling the plasmonic photothermal properties of the metal nanoparticles for plasmonic hyperthermia and the chemoaction of drug DOXO for potential intended combinatorial cancer therapeutics in the future as well as providing different strategies for the controlled and sustained release of the cargo drug molecules. To do that, DOXO could be successfully loaded onto the hybrid nanoconstructs through electrostatic interactions with high efficiencies of up to ca. 78.3 ± 6.9% for the first formed drug layer and 56 ± 13% for the second one, with a total efficiency for the whole system [(PSS/DOXO/PLL)2/HA-coated NRs] of ca. 65.7 ± 1.4%. Nanohybrid internalization was observed to be enhanced by the outer HA layer, which is able to target the CD44 receptors widely overexpressed in some types of cancers as lung, breast, or ovarian ones. Hence, these nanohybrid systems might be versatile nanoplatforms to simultaneously deliver sufficient heat for therapeutic plasmonic hyperthermia and the anticancer drug. Two controlled mechanisms were proposed to modulate the release of the chemodrug, one by means of the enzymatic degradable character of the PLL layer and another by the modulation of the interactions between the polymeric layers through the exploitation of the optical properties of the hybrid particles under near infrared (NIR) laser irradiation. The combination of this bimodal therapeutic approach exerted a synergistic cytotoxic effect on both HeLa and MDA-MB-231 cancer cells in vitro. Cell death mechanisms were also analyzed, elucidating that plasmonic photothermal therapy induces cell necrosis, whereas DOXO activates the cell apoptotic pathway. Therefore, the present NIR laser-induced targeted cancer thermo/chemotherapy represents a novel targeted anticancer strategy with easy control on demand and suitable therapeutic efficacy.

Dataset Information

Targeted highly sensitive detection/eradication of multi-drug resistant Salmonella DT104 through gold nanoparticle-SWCNT bioconjugated nanohybrids.

Publications

Targeted highly sensitive detection/eradication of multi-drug resistant Salmonella DT104 through gold nanoparticle-SWCNT bioconjugated nanohybrids.

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