Unknown

Dataset Information

0

Isolation and molecular characterization of circulating melanoma cells.


ABSTRACT: Melanoma is an invasive malignancy with a high frequency of blood-borne metastases, but circulating tumor cells (CTCs) have not been readily isolated. We adapted microfluidic CTC capture to a tamoxifen-driven B-RAF/PTEN mouse melanoma model. CTCs were detected in all tumor-bearing mice and rapidly declined after B-RAF inhibitor treatment. CTCs were shed early from localized tumors, and a short course of B-RAF inhibition following surgical resection was sufficient to dramatically suppress distant metastases. The large number of CTCs in melanoma-bearing mice enabled a comparison of RNA-sequencing profiles with matched primary tumors. A mouse melanoma CTC-derived signature correlated with invasiveness and cellular motility in human melanoma. CTCs were detected in smaller numbers in patients with metastatic melanoma and declined with successful B-RAF-targeted therapy. Together, the capture and molecular characterization of CTCs provide insight into the hematogenous spread of melanoma.

SUBMITTER: Luo X 

PROVIDER: S-EPMC4079008 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature

altmetric image

Publications


Melanoma is an invasive malignancy with a high frequency of blood-borne metastases, but circulating tumor cells (CTCs) have not been readily isolated. We adapted microfluidic CTC capture to a tamoxifen-driven B-RAF/PTEN mouse melanoma model. CTCs were detected in all tumor-bearing mice and rapidly declined after B-RAF inhibitor treatment. CTCs were shed early from localized tumors, and a short course of B-RAF inhibition following surgical resection was sufficient to dramatically suppress distant  ...[more]

Similar Datasets

2014-03-31 | E-GEOD-52031 | biostudies-arrayexpress
2014-03-31 | GSE52031 | GEO
| 46798 | ecrin-mdr-crc
| S-EPMC5700979 | biostudies-literature
2017-10-08 | GSE104209 | GEO
| S-EPMC6368301 | biostudies-literature
| S-EPMC4903174 | biostudies-literature
| S-EPMC5864750 | biostudies-literature
| S-EPMC9797203 | biostudies-literature
| PRJNA730416 | ENA