Unknown

Dataset Information

0

Invariant natural killer T cells recognize a fungal glycosphingolipid that can induce airway hyperreactivity.


ABSTRACT: Aspergillus fumigatus is a saprophytic fungus that is ubiquitous in the environment and is commonly associated with allergic sensitization and severe asthma in humans. Although A. fumigatus is recognized by multiple microbial pattern-recognition receptors, we found that an A. fumigatus-derived glycosphingolipid, asperamide B, directly activates invariant natural killer T (iNKT) cells in vitro in a CD1d-restricted, MyD88-independent and dectin-1-independent fashion. Moreover, asperamide B, when loaded onto CD1d, directly stained, and was sufficient to activate, human and mouse iNKT cells. In vivo, asperamide B rapidly induced airway hyperreactivity, which is a cardinal feature of asthma, by activating pulmonary iNKT cells in an interleukin-33 (IL-33)-ST2-dependent fashion. Asperamide B is thus the first fungal glycolipid found to directly activate iNKT cells. These results extend the range of microorganisms that can be directly detected by iNKT cells to the kingdom of fungi and may explain how A. fumigatus can induce severe chronic respiratory diseases in humans.

SUBMITTER: Albacker LA 

PROVIDER: S-EPMC4079117 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Invariant natural killer T cells recognize a fungal glycosphingolipid that can induce airway hyperreactivity.

Albacker Lee A LA   Chaudhary Vinod V   Chang Ya-Jen YJ   Kim Hye Young HY   Chuang Ya-Ting YT   Pichavant Muriel M   DeKruyff Rosemarie H RH   Savage Paul B PB   Umetsu Dale T DT  

Nature medicine 20130901 10


Aspergillus fumigatus is a saprophytic fungus that is ubiquitous in the environment and is commonly associated with allergic sensitization and severe asthma in humans. Although A. fumigatus is recognized by multiple microbial pattern-recognition receptors, we found that an A. fumigatus-derived glycosphingolipid, asperamide B, directly activates invariant natural killer T (iNKT) cells in vitro in a CD1d-restricted, MyD88-independent and dectin-1-independent fashion. Moreover, asperamide B, when l  ...[more]

Similar Datasets

| S-EPMC3178673 | biostudies-literature
| S-EPMC3242449 | biostudies-literature
| S-EPMC4466557 | biostudies-literature
| S-EPMC2118110 | biostudies-literature
| S-EPMC1413796 | biostudies-literature
| S-EPMC6138764 | biostudies-literature
| S-EPMC4980213 | biostudies-other
| S-EPMC8491998 | biostudies-literature
| S-EPMC4450678 | biostudies-literature
| S-EPMC2271004 | biostudies-literature