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Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana.

ABSTRACT: A novel Gram-stain-positive, facultatively anaerobic, non-motile and lactic-acid-producing bacterium, designated strain ORL-24(T), was isolated from the gut of the camel cricket, Diestrammena coreana. Optimal growth occurred at 37 °C, pH 8 and with 0?% (w/v) NaCl. The ratio of l-lactate to d-lactate in strain ORL-24(T) was 96?:?4. Lancefield antigen D was not detected. The strain was negative for oxidase activity and catalase activity. According to a phylogenetic analysis based on 16S rRNA gene sequences, strain ORL-24(T) was most closely related to the type strain of Enterococcus asini (96.9?% similarity). Comparative pheS and rpoA sequence analyses of strain ORL-24(T) indicated that the strain belonged to the genus Enterococcus. The major fatty acids were C16?:?0 and C18?:?1?9c. The DNA G+C content was 41.3 mol%. Based on phenotypic, genotypic and phylogenetic analyses, strain ORL-24(T) represents a novel species of the genus Enterococcus, for which the name Enterococcus diestrammenae is proposed. The type strain is ORL-24(T) (?=?KACC 16708(T)?=?JCM 18359(T)).

SUBMITTER: Kim JY

PROVIDER: S-EPMC4080732 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature

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Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana.

Kim Joon Yong JY Shin Na-Ri NR Na Hong-Kyung HK Hyun Dong-Wook DW Whon Tae Woong TW Kim Pil Soo PS Yun Ji-Hyun JH Bae Jin-Woo JW

International journal of systematic and evolutionary microbiology 20130801 Pt 12

A novel Gram-stain-positive, facultatively anaerobic, non-motile and lactic-acid-producing bacterium, designated strain ORL-24(T), was isolated from the gut of the camel cricket, Diestrammena coreana. Optimal growth occurred at 37 °C, pH 8 and with 0 % (w/v) NaCl. The ratio of l-lactate to d-lactate in strain ORL-24(T) was 96 : 4. Lancefield antigen D was not detected. The strain was negative for oxidase activity and catalase activity. According to a phylogenetic analysis based on 16S rRNA gene ...[more]

PMID: 23907226

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Project description:As a reference laboratory, the Streptococcus Laboratory at the Centers for Disease Control and Prevention (CDC) is frequently asked to confirm the identity of unusual or difficult-to-identify catalase-negative, gram-positive cocci. In order to accomplish the precise identification of these microorganisms, we have systematically applied analysis of whole-cell protein profiles (WCPP) and DNA-DNA reassociation experiments, in conjunction with conventional physiological tests. Using this approach, we recently focused on the characterization of three strains resembling the physiological groups I (strain SS-1730), II (strain SS-1729), and IV (strain SS-1728) of enterococcal species. Two strains were isolated from human blood, and one was isolated from human brain tissue. The results of physiological testing were not consistent enough to allow confident inclusion of the strains in any of the known enterococcal species. Resistance to vancomycin was detected in one of the strains (SS-1729). Analysis of WCPP showed unique profiles for each strain, which were not similar to the profiles of any previously described Enterococcus species. 16S ribosomal DNA (rDNA) sequencing results revealed three new taxa within the genus ENTEROCOCCUS: The results of DNA-DNA relatedness experiments were consistent with the results of WCPP analysis and 16S rDNA sequencing, since the percentages of homology with all 25 known species of Enterococcus were lower than 70%. Overall, the results indicate that these three strains constitute three new species of Enterococcus identified from human clinical sources, including one that harbors the vanA gene. The isolates were provisionally designated Enterococcus sp. nov. CDC Proposed New Species of Enterococcus 1 (CDC PNS-E1), type strain SS-1728(T) (= ATCC BAA-780(T) = CCUG 47860(T)); Enterococcus sp. nov. CDC PNS-E2, type strain SS-1729(T) (= ATCC BAA-781(T) = CCUG 47861(T)); and Enterococcus sp. nov. CDC PNS-E3, type strain SS-1730(T) (= ATCC BAA-782(T) = CCUG 47862(T)).

Dataset Information

Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana.

Publications

Enterococcus diestrammenae sp. nov., isolated from the gut of Diestrammena coreana.

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