Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells.
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ABSTRACT:
SUBMITTER: Rajesh D
PROVIDER: S-EPMC4081297 | biostudies-literature | 2011 Aug
REPOSITORIES: biostudies-literature
Publications
Human lymphoblastoid B-cell lines reprogrammed to EBV-free induced pluripotent stem cells.
Rajesh Deepika D Dickerson Sarah J SJ Yu Junying J Brown Matthew E ME Thomson James A JA Seay Nicholas J NJ
Blood 20110627 7
Generation of patient-specific induced pluripotent cells (iPSCs) holds great promise for regenerative medicine. Epstein-Barr virus immortalized lymphoblastoid B-cell lines (LCLs) can be generated from a minimal amount of blood and are banked worldwide as cellular reference material for immunologic or genetic analysis of pedigreed study populations. We report the generation of iPSCs from 2 LCLs (LCL-iPSCs) via a feeder-free episomal method using a cocktail of transcription factors and small molec ...[more]
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