Ontology highlight
ABSTRACT:
SUBMITTER: Shiomi H
PROVIDER: S-EPMC4081812 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
PloS one 20140703 7
This paper describes the utilization of giant unilamellar vesicles (GUVs) as a platform for handling chemical and biochemical reagents. GUVs with diameters of 5 to 10 µm and containing chemical/biochemical reagents together with inert polymers were fused with electric pulses (electrofusion). After reagent mixing, the fused GUVs spontaneously deformed to a budding shape, separating the mixed solution into sub-volumes. We utilized a microfluidic channel and optical tweezers to select GUVs of inter ...[more]