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Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1).


ABSTRACT: Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum coverage of five and 90% of the reads mapped on the target regions. Additionally, it is shown that the reproducibility within and between captures is high and that pooling four samples per capture is a valid option. Overall, we have demonstrated the strong performance of this WES enrichment kit and are confident it will be a valuable tool in future disease association studies.

SUBMITTER: Broeckx BJ 

PROVIDER: S-EPMC4083258 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature

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Development and performance of a targeted whole exome sequencing enrichment kit for the dog (Canis Familiaris Build 3.1).

Broeckx Bart J G BJ   Coopman Frank F   Verhoeven Geert E C GE   Bavegems Valérie V   De Keulenaer Sarah S   De Meester Ellen E   Van Niewerburgh Filip F   Deforce Dieter D  

Scientific reports 20140707


Whole exome sequencing is a technique that aims to selectively sequence all exons of protein-coding genes. A canine whole exome sequencing enrichment kit was designed based on the latest canine reference genome (build 3.1.72). Its performance was tested by sequencing 2 exome captures, each consisting of 4 pre-capture pooled, barcoded Illumina libraries on an Illumina HiSeq 2500. At an average sequencing depth of 102x, 83 to 86% of the target regions were completely sequenced with a minimum cover  ...[more]

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