Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria.
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ABSTRACT:
SUBMITTER: Hempel C
PROVIDER: S-EPMC4083392 | biostudies-literature | 2014 Jul
REPOSITORIES: biostudies-literature
Publications
Endothelial glycocalyx on brain endothelial cells is lost in experimental cerebral malaria.
Hempel Casper C Hyttel Poul P Kurtzhals Jørgen A L JA
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 20140423 7
We hypothesized that the glycocalyx, which is important for endothelial integrity, is lost in severe malaria. C57BL/6 mice were infected with Plasmodium berghei ANKA, resulting in cerebral malaria, or P. chabaudi AS, resulting in uncomplicated malaria. We visualized the glycocalyx with transmission electron microscopy and measured circulating glycosaminoglycans by dot blot and ELISA. The glycocalyx was degraded in brain vasculature in cerebral and to a lesser degree uncomplicated malaria. It was ...[more]
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