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Combined treatment of isoflavone supplementation and exercise restores the changes in hepatic protein expression in ovariectomized rats - a proteomics approach.

ABSTRACT: BACKGROUND: Postmenopausal women experience adverse physiological changes caused by estrogen deprivation. Here, we hypothesized that the administration of isoflavone, a phytoestrogn, and/or physical exercise could reverse changes in the levels of hepatic enzymes disturbed by loss of estrogen to ameliorate postmenopause-related health problems. METHODS: Thirty-week-old female Sprague-Dawley rats were divided into five groups: a sham-operated (SHAM) group, ovariectomized groups on a regular diet with exercise (EXE) and without exercise (OVX), and ovariectomized groups on an isoflavone supplemented diet with (ISO?+?EXE) and without exercise (ISO). Proteomic tools were employed to identify candidate hepatic proteins that were differentially expressed among the five animal groups. RESULTS: INMT was detected in the SHAM but not in all of the ovariectomized rats. Seven proteins (PPIA, AKR1C3, ALDH2, PSME2, BUCS1, OTC, and GAMT) were identified to have differential expression among the groups. When compared to the SHAM group, the ovariectomy elevated the levels of PPIA, BUCS1, PSME2, AKR1C3, and GAMT while decreasing ALDH2 and OTC. Among these OVX-induced changes, OVX-increased BUCS1 and GAMT levels were noticeably decreased by ISO or EXE and further greatly down-regulated by ISO?+?EXE. In the case of PSME2, ISO and EXE further increased OVX-upregulated expression levels but ISO?+?EXE greatly reduced OVX-increased levels. On the other hand OVX-lowered OTC levels were elevated by ISO, EXE, or ISO?+?EXE. The protein levels of ALDH2, PPIA, and AKR1C3 were not significantly reverted by ISO, EXE or ISO?+?EXE. CONCLUSION: The combination of an isoflavone diet and exercise partly reversed ovariectomy-induced changes in hepatic protein expression levels. Our data suggest that the combinatory regimen of isoflavone supplementation and exercise may be beneficial to menopausal women through modulating hepatic protein expression profiles.

SUBMITTER: Yoon S

PROVIDER: S-EPMC4085729 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature

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Combined treatment of isoflavone supplementation and exercise restores the changes in hepatic protein expression in ovariectomized rats - a proteomics approach.

Yoon Sun S Lee Joomin J Lee Seung-Min SM

Journal of the International Society of Sports Nutrition 20140614

<h4>Background</h4>Postmenopausal women experience adverse physiological changes caused by estrogen deprivation. Here, we hypothesized that the administration of isoflavone, a phytoestrogn, and/or physical exercise could reverse changes in the levels of hepatic enzymes disturbed by loss of estrogen to ameliorate postmenopause-related health problems.<h4>Methods</h4>Thirty-week-old female Sprague-Dawley rats were divided into five groups: a sham-operated (SHAM) group, ovariectomized groups on a r ...[more]

PMID: 25006332

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Project description:Background:The prevalence of nonalcoholic steatohepatitis (NASH) in menopausal women is increasing, but current treatments have not been proven effective. The objective of this study was to investigate the treatment effects of genistein and running exercise in ovariectomized (OVX) rats with NASH.Methods:Thirty-six female Sprague-Dawley rats were divided into 6 groups, control; OVX with standard diet; OVX with high fat and high fructose (HFHF) diet for 4?weeks; OVX with HFHF and genistein treatment (16?mg/kg BW/day) for 5?weeks (OVX?+?HFHF+GEN); OVX with HFHF and moderate intensity exercise for 5?weeks (OVX?+?HFHF+EX); OVX with HFHF and combined treatments (OVX?+?HFHF+GEN?+?EX). Serum interleukin-6 (IL-6) levels, hepatic free fatty acid (FFA), hepatic glutathione (GSH), and hepatic malondialdehyde (MDA) levels were measured. Liver histology was examined to determine NASH severity.Results:OVX?+?HFHF group had the highest levels of hepatic FFA compared with OVX and control groups (5.92?±?0.84 vs. 0.37?±?0.01 vs. 0.42?±?0.04?nmol/mg protein, respectively, p?<?0.01). Serum IL-6 levels were significantly elevated in both OVX and OVX?+?HFHF groups as compared with controls (112.13?±?6.50 vs. 121.47?±?3.96 vs. 86.13?±?2.40?pg/mL, respectively, p?<?0.01). In OVX?+?HFHF group, hepatic MDA levels were higher, while GSH levels were lower than in OVX and control groups (MDA; 0.98?±?0.04 vs. 0.82?±?0.02 vs. 0.78?±?0.03?nmol/mg protein, and GSH; 46.01?±?0.91 vs. 55.21?±?1.40 vs. 57.94?±?0.32, respectively; p?<?0.01 for both). Comparing with OVX?+?HFHF group, rats that received genistein, exercise and combined treatments demonstrated an improvement in liver histopathology, decreased levels of hepatic FFA (1.44?±?0.21 vs. 0.45?±?0.04 vs. 0.49?±?0.05?nmol/mg protein, respectively, p?<?0.01), serum IL-6 (82.80?±?2.07 vs. 83.47?±?2.81 vs. 94.13?±?1.61?pg/mL, respectively, p?<?0.01), and hepatic MDA (0.80?±?0.03 vs. 0.76?±?0.02 vs. 0.76?±?0.03?nmol/mg protein, respectively, p?<?0.01).Conclusions:Genistein and moderate intensity exercise were effective in reducing the severity of NASH in OVX rats through the reduction in liver inflammation, oxidative stress and liver fat contents.

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Combined treatment of isoflavone supplementation and exercise restores the changes in hepatic protein expression in ovariectomized rats - a proteomics approach.

Publications

Combined treatment of isoflavone supplementation and exercise restores the changes in hepatic protein expression in ovariectomized rats - a proteomics approach.

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