Ontology highlight
ABSTRACT:
SUBMITTER: Baek ST
PROVIDER: S-EPMC4086250 | biostudies-literature | 2014 Jun
REPOSITORIES: biostudies-literature
Baek Seung Tae ST Kerjan Geraldine G Bielas Stephanie L SL Lee Ji Eun JE Fenstermaker Ali G AG Novarino Gaia G Gleeson Joseph G JG
Neuron 20140601 6
Acute gene inactivation using short hairpin RNA (shRNA, knockdown) in developing brain is a powerful technique to study genetic function; however, discrepancies between knockdown and knockout murine phenotypes have left unanswered questions. For example, doublecortin (Dcx) knockdown but not knockout shows a neocortical neuronal migration phenotype. Here we report that in utero electroporation of shRNA, but not siRNA or miRNA, to Dcx demonstrates a migration phenotype in Dcx knockouts akin to the ...[more]