Dataset Information

Intravascular optical coherence tomography detection of atherosclerosis and inflammation in murine aorta.

ABSTRACT: The goal of this study was to evaluate the feasibility of imaging the aorta of apolipoprotein E-deficient (ApoE(-/-)) mice for the detection of atherosclerosis and macrophages using optical coherence tomography (OCT) compared with histology.Atherosclerosis was induced by high-fat diet in 7-week-old ApoE(-/-) mice for 10 (n=7) and 22 (n=7) weeks. Nine-week-old ApoE(-/-) mice (n=7) fed a standard chow diet were used as controls. OCT images of a 10-mm descending aorta in situ were performed in 4 mice for each, and plaque and macrophages were determined at 0.5-mm intervals. Automated detection and quantification of macrophages were performed independently using a customized algorithm. Coregistered histological cross-sections were stained with hematoxylin-eosin, Mac-3, and von Kossa. Three mice in each group had en face OCT imaging to detect macrophages, which were compared with lipid-positive area with Sudan IV. OCT images were successfully acquired in all mice. OCT and histology were able to discriminate macrophages and plaque among the 3 groups and showed excellent correlation for (1) visual detection of plaque (r=0.98) and macrophages (r=0.93), (2) automated detection and quantification of macrophages by OCT versus Mac-3-positive area (r=0.92), and (3) en face OCT detection of macrophages versus Sudan IV-positive area (r=0.92).Murine intra-aortic OCT is feasible and shows excellent correlation with histology for detection of atherosclerotic plaque and macrophages.

SUBMITTER: Tahara S

PROVIDER: S-EPMC4091044 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature

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Intravascular optical coherence tomography detection of atherosclerosis and inflammation in murine aorta.

Tahara Satoko S Morooka Toshifumi T Wang Zhao Z Bezerra Hiram G HG Rollins Andrew M AM Simon Daniel I DI Costa Marco A MA

Arteriosclerosis, thrombosis, and vascular biology 20120202 5

<h4>Objective</h4>The goal of this study was to evaluate the feasibility of imaging the aorta of apolipoprotein E-deficient (ApoE(-/-)) mice for the detection of atherosclerosis and macrophages using optical coherence tomography (OCT) compared with histology.<h4>Methods and results</h4>Atherosclerosis was induced by high-fat diet in 7-week-old ApoE(-/-) mice for 10 (n=7) and 22 (n=7) weeks. Nine-week-old ApoE(-/-) mice (n=7) fed a standard chow diet were used as controls. OCT images of a 10-mm d ...[more]

PMID: 22308042

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Dataset Information

Intravascular optical coherence tomography detection of atherosclerosis and inflammation in murine aorta.

Publications

Intravascular optical coherence tomography detection of atherosclerosis and inflammation in murine aorta.

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