Ontology highlight
ABSTRACT:
SUBMITTER: Epp E
PROVIDER: S-EPMC4092012 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Epp Elias E Walther Andrea A Lépine Guylaine G Leon Zully Z Mullick Alaka A Raymond Martine M Wendland Jürgen J Whiteway Malcolm M
Molecular microbiology 20100204 5
Candida albicans is a diploid fungal pathogen lacking a defined complete sexual cycle, and thus has been refractory to standard forward genetic analysis. Instead, transcription profiling and reverse genetic strategies based on Saccharomyces cerevisiae have typically been used to link genes to functions. To overcome restrictions inherent in such indirect approaches, we have investigated a forward genetic mutagenesis strategy based on the UAU1 technology. We screened 4700 random insertion mutants ...[more]